Section | Primer (5′-3′) | Beginning nucleotide | End nucleotide | Length (bp) | Tm |
---|---|---|---|---|---|
I | GACAATGACCGAAGACTC | 17 | 693 | 677 | 46 °C |
ATGCTAATGCTATTACTACG | |||||
II | GCGAGGTTTCATATTACAA | 614 | 1262 | 649 | 49 °C |
ACCCAAGAAGATAATCACAA | |||||
III | CGTGGCACATGCTCTTCT | 1222 | 1833 | 612 | 55 °C |
TGACCGCGTTCGAGTCAT | |||||
IV | CACAAGAACATTTGCCGTAC | 1792 | 2401 | 610 | 53 °C |
TCCTGGAACTTGAGCCAC | |||||
V | GGCTCAAGTTCCAGGAGT | 2386 | 3012 | 627 | 51 °C |
CACCCACAAGCATACAATC | |||||
VI | GTGGGATTGTATGCTTGTG | 2990 | 3592 | 603 | 52 °C |
ATGCCTCTATGATTCAGTTCGT | |||||
VII | CCAAGGTGATAGGCAATT | 3520 | 4156 | 637 | 51 °C |
CAGGCGTTCGTAAAGTAAA | |||||
VIII | CAAGTGGTTGGCGCTGGGTT | 4112 | 4768 | 657 | 63 °C |
CCGGCTTTCTTCTTCTGTTCGT | |||||
IX | TTCATCATCTTCGGGTCG | 4683 | 5326 | 644 | 52 °C |
AAGAACAGCAGCAGACAGA | |||||
X | GTCAAGGGTGCCAAAGGT | 5238 | 5914 | 677 | 55 °C |
TTCCGAGCGAAGAAGTCC | |||||
XI | ACATACCGATCTGTCGAG | 5830 | 6495 | 660 | 47 °C |
ATTTCTGTCGTGCTTCTG |