Fig. 2

Effects of L-Arg treatment on NO (a) and ROS (b) production in spleen cells. a Supernatants were prepared from splenocyte cultures, and concentrations of NO₂ ⁻ were detected using the Griess reaction. b ROS production was measured by flow cytometry. Spleen cells were collected from mice prior to Py17XL infection (0 d), and 3 and 5 dpi. Results are representative of three independent experiments. For each experiment, three mice were used per group. Values represent the mean + SEM. * indicates significant difference (P < 0.05, t-test) between Py17XL infected (3 d and 5 d) and uninfected mice (0 d). # indicates significant difference (P < 0.05, t-test) between D-Arg pretreated control (Normal infection) and L-Arg pretreated mice (L-Arg treatment)