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Table 1 Distribution and relative staining intensity for Nras/let-60 in untreated and ivermectin-treated O. volvulus adult female worms

From: A histochemical study of the Nras/let-60 activity in filarial nematodes

Anatomical location

Untreated parasites

Ivermectin treated parasites

 

Uniformitya

Intensityb

Uniformitya

Intensityb

Hypodermis

3

3

2

1

Uterine epithelium

3

3

1

1

Intestinal epithelium

2

2

2

1

Oocytes

3

3

3

1

Morulae

3

3

NP

NP

Coiled mff

3

3

NP

NP

Mature mff

3

3

NP

NP

Spermatocytes

3

3

2

1

Spermatozoa

1

1

1

1

  1. aUniformity score: Based on the overall distribution of the staining throughout the particular anatomical structure. Range: 3 = predominantly present; 2 = moderately present; 1 = limited presence; 0 = no staining present, NP = stage not present in worm
  2. bIntensity Score: Based on the overall intensity observed in all positive locations compared to human tissue control in the same section. Range: 0 = no staining; 1 = weak staining; 2 = moderate staining; 3 = strong staining