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Fig. 2 | Parasites & Vectors

Fig. 2

From: A Leishmania-specific hypothetical protein expressed in both promastigote and amastigote stages of Leishmania infantum employed for the serodiagnosis of, and as a vaccine candidate against, visceral leishmaniasis

Fig. 2

Immune response induced by immunization with rLiHyV protein plus saponin. Single cells suspensions were obtained from the spleen of mice, four weeks after the last immunization and before infection. Cells were unstimulated (negative control), or separately stimulated with rLiHyV (rLiHyV/saponin group, 20 μg/mL), Peptide1 (Peptide1/saponin group, 20 μg/mL) or Peptide2 (Peptide2/saponin group, 25 μg/mL). Saline and saponin groups were stimulated with SLA (25 μg/mL). IFN-γ, IL-12, GM-CSF, IL-4, and IL-10 levels were measured by a capture ELISA (a). Cytokine levels of spleen cells derived from animals immunized with rLiHyV/saponin and stimulated with rLiHyV, Peptide1, Peptide2 or SLA (b). Statistically significant differences (P < 0.001) were observed between negative control (medium), SLA, Peptide1 and Peptide2 (a, b, c, and d, respectively) and in ratios of IFN-γ/IL-10 and IFN-γ/IL-4 (c), as well as between IL-12/IL-10 and IL-12/IL-4 (d). Bars represent the mean ± standard deviation of the groups. Statistically significant differences (P < 0.05) in relation to the saline, saponin or rLiHyV/saponin groups were observed (a, b and c respectively)

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