Fig. 3From: A Leishmania-specific hypothetical protein expressed in both promastigote and amastigote stages of Leishmania infantum employed for the serodiagnosis of, and as a vaccine candidate against, visceral leishmaniasisHumoral response induced by vaccination with rLiHyV protein plus saponin. Sera samples were collected from animals four weeks after the third and last immunization, and before infection. The parasite-specific IgG1 (a) and IgG2a (b) isotype levels were determined, and the bars represent the mean ± standard deviation of the groups. The ratios between IgG2a and IgG1 levels were also calculated (c). Statistically significant differences in relation to the saline, saponin or rLiHyV/saponin groups were observed (a, b and c letters, respectively; P < 0.05)Back to article page