Fig. 6From: A Leishmania-specific hypothetical protein expressed in both promastigote and amastigote stages of Leishmania infantum employed for the serodiagnosis of, and as a vaccine candidate against, visceral leishmaniasisAnalysis of humoral response after L. infantum infection. Sera samples were collected of animals, 10 weeks after infection, when the parasite-specific IgG1 (a) and IgG2a (b) isotype levels were evaluated. The ratios between the IgG2a and IgG1 levels were calculated, and the bars represent the mean ± standard deviation of the groups (c). Statistically significant differences in relation to the saline, saponin or rLiHyV/saponin groups were observed (a, b and c letters, respectively; P < 0.05)Back to article page