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Fig. 3 | Parasites & Vectors

Fig. 3

From: Exposure to Leishmania spp. and sand flies in domestic animals in northwestern Ethiopia

Fig. 3

Specificity of the anti-sand fly saliva IgG antibody response. The reactivity of Phlebotomus orientalis salivary gland homogenates (SGH) with sera from mice (a) and dogs (b) repeatedly exposed to a single sand fly species was measured via ELISA. In graph A, SGH was incubated with sera from mice exposed to P. orientalis (Anti-ORI), Sergentomyia schwetzi (Anti-SCHW), P. papatasi (Anti-PAP), P. duboscqi (Anti-DUB), or P. arabicus (Anti-ARA). Each bar represents the mean for two serum samples ± the standard error. The values for the positive controls (the sera of mice incubated with homologous antigen) were as follows: S. schwetzi = 1.48 ± 0.43, P. papatasi = 2.38 ± 0.32, P. duboscqi = 2.87 ± 0.60, and P. arabicus = 1.86 ± 0.24. In graph B, the SGH was incubated with the sera from dogs exposed to P. perniciosus (Anti-PER) or Lutzomyia longipalpis (Anti-LON). Seropositive Ethiopian dogs (Ethiopia) and dogs that had never been exposed to sand flies (neg) were used as positive and negative controls, respectively. Each bar represents the mean of five serum samples ± the standard error. The absorbencies of the sera incubated with the homologous antigen were 2.42 ± 0.06 for L. longipalpis and 1.73 ± 0.13 for P. perniciosus

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