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Fig. 4 | Parasites & Vectors

Fig. 4

From: Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

Fig. 4

Expression pattern of the EnOWP2-mCherry-reporter (construct 2) during oocyst sporulation. a: Freshly harvested oocyst with YFP but absent mCherry signal. b: Oocyst after 12 h sporulation with cytoplasmatic and nuclear YFP signal and weak extracytoplasmatic mCherry signal in the structureless space between the condensed cytoplasm and the oocyst wall, designated as ‘circumplasm’. c: Oocyst after 17 h sporulation with strong mCherry signal in the circumplasm. d: Oocyst after 18 h during first meiotic reduction division. e: Sporulating oocyst during sporoblast formation. f: Sporulating oocyst after sporoblast formation at 24 h sporulation. g: Sporulating oocyst after. 26 h sporulation with mCherry signals in the circumplasm and protein accumulation at one end of the sporocyst. h: Full sporulated oocyst after 4 d sporulation. The mCherry signal surround the YFP signal and co-localise apparently with the sporocyst wall. Scale bar 5 μm. TL: transmitted light; UV: UV autofluorescence of the oocyst wall; YFP: YFP fluorescence; mCherry: mCherry fluorescence; MCF (multichannel fluorescence) overlay of all fluorescent signals in the row

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Parasites & Vectors

ISSN: 1756-3305

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