Fig. 6From: Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formationImmunofluorescence detection of mCherry in the sporocyst wall of mCherry positive but YFP negative sporocysts with goat anti-mCherry antibody and secondary anti-goat FITC antibody. a: Methanol fixed and Triton treated sporocyst incubated with primary anti-mCherry antibody and secondary anti-goat FITC antibody shows the signal of mCherry and the FITC signal of the antibody interaction with the sporocyst. b: Same antibody combination like in a but without Methanol fixation and Triton treatment shows similar mCherry signal, but a much reduced FITC signal. c: Methanol fixed sporocyst with Triton X100 treatment, without primary (anti mCherry) antibody but with secondary anti-goat FITC antibody does not show green fluorescent signal (K). Scale bar 5 μm. TL: Transmitted light micrograph, mCherry: mCherry signal of the sporocyst FITC: Signal of the anti-Goat FITC AntibodyBack to article page