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Fig. 8 | Parasites & Vectors

Fig. 8

From: Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

Fig. 8

Fluorescence pattern of EnOWP2Exon1-mCherry (construct 3). a: Freshly harvested oocyst with cytoplasmatic YFP but absent mCherry signal. b: Oocyst after 16 h sporulation with cytoplasmatic YFP signal and weak intra- and extracytoplasmatic mCherry signal. Red fluorescent vesicle-like signals are visible at the border of the cytoplasm. c: oocyst at the end of first meiotic division at 26 h sporulation d: oocyst during sporoblast formation with cytoplasmatic and nuclear associated YFP signal, as well as extracytoplasmatic mCherry signal, vesicle-like mCherry signal are in the sporoblast are visible as well ; 26 h sporulation period. e: oocyst with sporoblast during sporozoite formation after 36 h sporulation period. The mCherry signals disappear from the extracytoplasmatic space and appear associated with the sporocyst wall. f: Fully sporulated oocyst after approx. 2d sporulation period. The mCherry signal surround the YFP signals close to the sporocyst wall and are also localised to the sporocyst residuum (granular signal). Scale bar 5 μm. TL: transmitted light; YFP: YFP fluorescence; mCherry: mCherry fluorescence; MCF (multichannel fluorescence) overlay of all fluorescent signals in the row

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Parasites & Vectors

ISSN: 1756-3305

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