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Table 1 Proportion of transgenic oocysts (at least one fluorochrome is visibly expressed) in the single passages of parasites transformed with the pDLY-EnOWP2-promoter_mC, pDLY_EnOWP2_mC, pDLY_EnOWP2Exon1_mC, and pDLY_EnOWP6_mC construct and selected by pyrimethamine

From: Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

Construct P Part of transformed oocystsa (trOoc/total) Part of mcherry + oocysts in trOoc* Part of yfp + oocysts in trOoc Proportion of YFP expressing sporocyst per transformed sporulated oocystb
   1 2 3 4
construct 1 P0 n.d. n.d. n.d. n.d n.d. n.d. n.d.
EnOWP2 promoter mCherry P1 50 % (26/52) 100 % (26) 100 % (26) 15 % 35 % 4 % 46 %
construct 2 P0 33 % (41/123) 95 % (39) 100 % (29) 12 % 24 % 7 % 56 %
EnOWP2-mCherry P1 42 % (75/180) 96 % (72) 100 % (75) 11 % 37 % 5 % 47 %
P2 56 % (74/131) 100 % (74) 100 % (74) 16 % 33 % 5 % 45 %
  P3 64 %(102/159) 100 % (102) 97 % (99) 20 % 27 % 6 % 43 %
construct 3 P0 n.d. n.d. n.d. n.d n.d. n.d. n.d.
EnOWP2-Exon1_ mCherry
  P1 26 % (45/170) 67 % (30) 100 % (45) 4 % 40 % 4 % 51 %
construct 4 P0 36 % (45/123) 42 % (high) 100 % (45) 9 % 29 % 7 % 55 %
EnOWP6 -mCherry (construct 4
  P1 21 % (25/120) 96 % (24) (46 % high 54 % low) 100 % (25) 16 % (4) mC low 16 % (4) mC low 8 % (2) mC low 60 % (15) mC high: 11 mC low: 3 no mC: 1)
  1. aat least one fluorochrome microscopically visible *
  2. brounded, n.d. not determined