Fig. 2

Effect of pH on the proteolytic activities of midgut extracts from C. quinquefasciatus females fed on sugar. a. The pH influence was evaluated by incubation of protein extracts at 37 °C for 12 h in 0.1 M sodium acetate buffer pH 3.5, 5.5 or 0.1 M Tris–HCl buffer pH 7.5, 10.0. The numbers on the left indicate the molecular mass of standards utilized in the gel (kDa). b. In-solution assays were performed using the fluorogenic substrate Z-Phe-Arg-AMC in 0.1 M sodium acetate buffer pH 3.5, 5.5 or 0.1 MTris–HCl buffer pH 7.5 or 10.0