Fig. 3

Effect of peptidase inhibitors on the proteolytic profiles of midgut extracts from C. quinquefasciatus females fed on sugar. a. Samples were pre-incubated for 30 min in the presence of 1 mM PMSF, 100 μM TLCK and 100 μM TPCK. The proteolytic activities were detected after incubating the gels for 12 h at 37 °C in Tris–HCl buffer (pH 7.5). The control was processed under the same conditions but in the absence of inhibitors. The numbers on the left indicate the molecular mass of standards utilized in the gel (kDa). b. The in-solution assays were performed using the fluorogenic substrate Z-Phe-Arg-AMC in 100 mM Tris-HCl buffer, pH 7.5, in the absence (control) or presence of 1 mM PMSF, 100 μM TLCK, 20 μM E-64 or 100 μM TPCK