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Table 3 In silico characterization of trypsin-like serine peptidases identified in the midgut of Culex quinquefasciatus females fed on sugar

From: In-depth characterization of trypsin-like serine peptidases in the midgut of the sugar fed Culex quinquefasciatus

Uniprot accession number

Protein name

Target P prediction â‘ 

Signal P prediction â‘¡

TMHMM prediction â‘¢

N-Glycosylation prediction â‘£

O-Glycosylation prediction ⑤

Exon number â‘¥

Paralogues number â‘¥

Supercontig â‘¥

B0WIS4

Trypsin 1

S (0.901)

23^24 (0.761)

No

158-NETV (0.7243)

36-T (0.627995)/40-S (0.689668)

1

36

3.14

B0WE94

Trypsin 2

S (0.910)

18^19 (0.818)

No

No

No

2

36

3.94

B0XCW2

Trypsin 4

S (0.973)

22^23 (0.935)

inside: 1-6/Tmhelix:7-26/outside: 27-258

27-NGTQ (0.8040)

No

2

36

3.91

B0X667

Trypsin 5

S (0.952)

17^18 (0.855)

inside: 291-293/Tmhelix: 268-290/outside: 1-267

65-NRTV (0.6702)/183-NVTV (0.8306)

151-T (0.653105)/159-S (0.523482)

3

13

3.59

B0XES8

Trypsin 7

S (0.832)

21^22 (0.665)

No

106-NVTF (0.6360)

No

3

36

3.11

B0X870

SP24D

S (0.891)

20^21 (0.801)

No

69-NGSV (0.6998)/75-NLSV (0.6183)

No

2

24

3.66

B0W9S9

Serine protease 1/2

S (0.960)

26^27 (0.750)

No

No

44-S (0.785129)

2

38

3.54

B0WW44

Cationic trypsin

S (0.926)

20^21 (0.794)

No

No

No

3

24

3.33

  1. ① TargetP 1.1 Server. Prediction of the subcellular location of trypsin. S = secreted. The number into the parenthesis indicates the probability
  2. â‘¡ SignalP 4.0 Server. Prediction of presence and location of signal peptide cleavage sites in the trypsin sequences. The numbers indicates the number of the amino acid residues involved in the cleavage. The number into the parenthesis indicates the probability
  3. â‘¢ TMHMM 2.0 Server. Prediction of transmembrane helices in proteins. Tmhelix: transmembrane helix
  4. â‘£ NetNglyc 1.0 Server. Prediction of N-Glycosylation sites based on the presence of Asn-Xaa-Ser/Thr motifs. The number into the parenthesis indicates the probability
  5. ⑤ NetOglyc 4.0 Server. Prediction of mucin type GalNAc O-glycosylation sites
  6. â‘¥ According to VectorBase database