Table 4 Proteotypic peptides from trypsins identified by MS/MS. Proteotypic peptides were predicted using SKYLINE software
Uniprot accession number | Protein name | Peptides identified by MS/MS | Species-specific peptide | Trypsin unique peptide | Other proteins witn the same peptide |
|---|---|---|---|---|---|
B0WIS4 | Trypsin 1 | R.AAYVPAYNQNQCNSAYAR.Y | Yes | No | Q1KWX6 (Trypsin-like fragment) - C. quinquefasciatus |
| Â | Â | R.IVGGFEISIADAPHQVSLQSR.G | Yes | Yes | - |
| Â | Â | R.GSHICGGSIISPK.W | Yes | Yes | - |
| Â | Â | R.NTIDYDYSLLELK.S | Yes | Yes | - |
| Â | Â | K.WILTAAHCTDGASVSNLR.I | Yes | Yes | - |
| Â | Â | K.HASGGSVISIK.R | - | - | Not predicted by Skyline |
B0WE94 | Trypsin 2 | R.DEPADESQSLVSGWGDTR.S | No | No | Q962G7 / Q56GM3 (Trypsin) - Culex pipiens |
| Â | Â | R.LEFGHAVQPVDLVR.D | No | No | Q962G7 / Q56GM3 (Trypsin) - Culex pipiens |
| Â | Â | R.GVLVPLVNR.E | No | No | Q962G7 / Q56GM3 (Trypsin) - Culex pipiens |
B0XCW2 | Trypsin 4 | K.GCAQPDYYGVYADVEK.A | Yes | Yes | - |
| Â | Â | K.DFDFALLR.L | Yes | Yes | - |
| Â | Â | R.VGSSYDYQGGTVIDVAGMTIHPR.Y | - | - | Not predicted by Skyline |
B0X667 | Trypsin 5 | K.IIGGFPAEQGDTLHQVSIR.F | Yes | Yes | - |
| Â | Â | K.GCGLAAYPGIYSDVAYYR.G | Yes | Yes | - |
| Â | Â | R.GWIDSCLAGK.C | Yes | Yes | - |
B0XES8 | Trypsin 7 | K.DACLGDSGGPLTCSGK.V | Yes | Yes | - |
| Â | Â | R.DYALLNLAK.S | Yes | Yes | - |
| Â | Â | R.AVDVPIADHDR.C | Yes | Yes | - |
| Â | Â | R.GGQLIAVTR.K | - | - | Not predicted by Skyline |
B0X870 | SP24D | K.LGESIEYDELSQPIALYEGDDLPK.D | - | - | Not predicted by Skyline |
B0W9S9 | Serine protease 1/2 | R.TGETFVDNQATVSGFGR.T | Yes | No | C. quinquefasciatus - Q23731 (Serine protease) |
| Â | Â | R.TVDGGPVSPTK.N | Yes | No | C. quinquefasciatus - Q23731 (Serine protease) |
B0WW44 | Cationic trypsin | R.IVVHPQYAEGNLANDIAVIR.V | Yes | Yes | - |