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Table 4 Proteotypic peptides from trypsins identified by MS/MS. Proteotypic peptides were predicted using SKYLINE software

From: In-depth characterization of trypsin-like serine peptidases in the midgut of the sugar fed Culex quinquefasciatus

Uniprot accession number Protein name Peptides identified by MS/MS Species-specific peptide Trypsin unique peptide Other proteins witn the same peptide
B0WIS4 Trypsin 1 R.AAYVPAYNQNQCNSAYAR.Y Yes No Q1KWX6 (Trypsin-like fragment) - C. quinquefasciatus
   R.IVGGFEISIADAPHQVSLQSR.G Yes Yes -
   R.GSHICGGSIISPK.W Yes Yes -
   R.NTIDYDYSLLELK.S Yes Yes -
   K.WILTAAHCTDGASVSNLR.I Yes Yes -
   K.HASGGSVISIK.R - - Not predicted by Skyline
B0WE94 Trypsin 2 R.DEPADESQSLVSGWGDTR.S No No Q962G7 / Q56GM3 (Trypsin) - Culex pipiens
   R.LEFGHAVQPVDLVR.D No No Q962G7 / Q56GM3 (Trypsin) - Culex pipiens
   R.GVLVPLVNR.E No No Q962G7 / Q56GM3 (Trypsin) - Culex pipiens
B0XCW2 Trypsin 4 K.GCAQPDYYGVYADVEK.A Yes Yes -
   K.DFDFALLR.L Yes Yes -
   R.VGSSYDYQGGTVIDVAGMTIHPR.Y - - Not predicted by Skyline
B0X667 Trypsin 5 K.IIGGFPAEQGDTLHQVSIR.F Yes Yes -
   K.GCGLAAYPGIYSDVAYYR.G Yes Yes -
   R.GWIDSCLAGK.C Yes Yes -
B0XES8 Trypsin 7 K.DACLGDSGGPLTCSGK.V Yes Yes -
   R.DYALLNLAK.S Yes Yes -
   R.AVDVPIADHDR.C Yes Yes -
   R.GGQLIAVTR.K - - Not predicted by Skyline
B0X870 SP24D K.LGESIEYDELSQPIALYEGDDLPK.D - - Not predicted by Skyline
B0W9S9 Serine protease 1/2 R.TGETFVDNQATVSGFGR.T Yes No C. quinquefasciatus - Q23731 (Serine protease)
   R.TVDGGPVSPTK.N Yes No C. quinquefasciatus - Q23731 (Serine protease)
B0WW44 Cationic trypsin R.IVVHPQYAEGNLANDIAVIR.V Yes Yes -