Multilocus microsatellite typing of Leishmania infantum isolates in monitored Leishmania/HIV coinfected patients

Table 2 Characteristics of the 20 microsatellite loci used in this study for Leishmania infantum genotyping

Locus	GenBank accession no.	Allele size (bp)	Dye label	Ta (°C)	Na	H _S	H _o
Li22-35^c	AM050045	90–106	VIC	58	5	0.191	0.042
Li45-24^c	AM050048	88–108	NED	58	5	0.208	0.060
Li71-5/2^c	AM050050	104–108	VIC	54	3	0.280	0.042
Li72-20^c	AM050057	87–95	VIC	50	4	0.275	0.042
LiBTA^a	nd	226–246	VIC	58	4	0.316	0.167
LiBTG^a	nd	219–257	6-FAM	58	7	0.340	0.104
LIST7021^b	AF427869	228–246	6-FAM	54	6	0.338	0.143
LIST7024^b	AF427872	198–224	VIC	59	3	0.049	0.000
LIST7025^b	AF427873	171–179	6-FAM	56	1	0.000	0.000
LIST7026^b	AF427874	201–231	NED	56	4	0.089	0.000
LIST7028^b	AF427876	104–108	VIC	58	2	0.049	0.000
LIST7031^b	AF427879	166–174	PET	54	1	0.000	0.000
LIST7033^b	AF427881	196–226	6-FAM	58	5	0.349	0.411
LIST7035^b	AF427883	188–202	PET	56	6	0.373	0.146
LIST7037^b	AF427885	178–194	6-FAM	58	5	0.320	0.411
LIST7038^b	AF427886	122–130	NED	56	3	0.088	0.000
LIST7039^b	AF427887	199–215	PET	58	5	0.128	0.143
Rossi1^d	X76394	104–110	6-FAM	59	3	0.100	0.000
Rossi2^d	X76393	140–160	VIC	57	6	0.335	0.185
TubCA^c	nd	74–84	6-FAM	58	2	0.100	0.000
Mean value					4	0.196	0.095

Ta: annealing temperature (thermocycling conditions); Na: number of alleles; Hs: Nei’s unbiased genetic diversity within subsamples; Ho: observed heterozygosity (Nei & Chesser, 1983 [17]); ^aBulle, 2002 [32]; Hide, 2013 [24]; ^bJamjoon, 2002 [33]; ^cOchsenreither, 2006 [11]; ^dRossi, 1994 [34]

ISSN: 1756-3305