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Fig. 2 | Parasites & Vectors

Fig. 2

From: Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples

Fig. 2

Comparison of detection sensitivity of L. infantum LAMP and PCR. Total DNA from L. infantum MCAN/CN/90/SC was serially diluted from 10 pg to 0.1 fg and amplified by LAMP and conventional PCR. a Visual appearance of LAMP products. b Agarose gel electrophoresis of LAMP products. c Agarose gel electrophoresis of PCR products. Lane M, 100-bp ladder; lanes 1–6, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg and 0.1 fg DNA, respectively; lane 7, water (negative control)

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