Table 2 Primer sequences for real-time and nested PCR protocols, along with sequence of positive control used for real time PCR reactions. Sequencing primers are also listed

 R330F^a

5′- CGTTCTTAACCCAGCTCACG - 3′

 R480RL^a

5′- GCCTGGAGGTWAYGTCC – 3′

  P. relictum – Pos. Control

5′- GGGAACAAACTGCCTCAAGACGTTCTTAACCAGCT

 (Accession # NC012426)

CACGCATCGCTTCTAACGGTGAACTCTCATTCCAA

TGGAACCTTGTTCAAGTTCAAATAGATTGGTAAGG

TATAGCGTTTACTATCGAATGAAACAATGTGTTCC

ACCGCTAGTGTTTGCTTCTAACATTCCATTGCTTAT

AACTGTATGGACGTAACCTCCAGGCAAAGAAAAT

GACCGGTC – 3′

 H332F^a

5′ - GAGAATTATGGAGYGGATGGTG - 3′

 HAEMNR2^b

5′ - AGAGGTGTAGCATATCTATCTAC- 3′

 H350F^a

5′ – GGTGTTTTAGATATATGCATGC - 3′

 HAEMR2^c

5′ - GCATTATCTGGATGTGATAATGGT - 3′

Nested PCR – Leucocytozoon

 HAEMNFI^d

5′ - CATATATTAAGAGAAITATGGAG - 3′

 HAEMNR3^d

5′ - ATAGAAAGATAAGAAATACCATTC - 3′

 L350F^e

5′ - GGTGTTTTAGATACTTA -3′

 L890R^e

5′ - TACAATATGTTGAGGTGTTTG - 3′

 FIFI^f

5′ – GGGTCAAATGAGTTTCTGG - 3′

 R2^f

5′ - GCTGTATCATACCCTAAAGG - 3′

Sequencing – Leucocytozoon

 L545F^e

5′ - ACAAATGAGTTTCTGGGGA - 3′

 L825R^e

5′ - GCAATTCCAAATAAACTTTGAA - 3′