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Fig. 3 | Parasites & Vectors

Fig. 3

From: Nitric oxide maintains cell survival of Trichomonas vaginalis upon iron depletion

Fig. 3

NO production is an arginine- and proteasome-dependent process. a NO levels in DIP (180 μM) and L-NMMA (1 and 3 mM) co-treated cells after 3 (white bars) and 6 h (gray bars) incubation. *** p < 0.001, compared with the DIP-only group. b Proteasome activities were measured in iron-rich (IR) and -deficient (ID) cultured cells by detecting the fluorescence signal intensity. * p < 0.05, compared with the IR group. c NO levels of DIP- and MG132-treated (1 and 3 μM) cells after 3 and 6 h of incubation. * (p < 0.05) and*** (p < 0.001), compared with the DIP-only group. d Co-treatment with MG132 and arginine (5 mM) in iron-deficient cells after 6 h of incubation.*** p < 0.001, based on fold-change between the indicated groups

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