NO maintains the hydrogenosomal membrane potential of iron-deficient T. vaginalis.
a The histograms indicate the red signal of the cells treated with DIP (180 μM), DIP-MG132 (10 μM), and DIP-MG132-arginine (5 mM). Red fluorescence represents a hydrogenosome with high membrane potential. The red fluorescence intensities of negative control group (CCCP treated cells, 50 μM) and experimental groups were detected by using flow cytometry after JC-1 staining. b Quantification data of the red fluorescence intensities (geometric mean) in panel a are shown. All tested groups were collected 6 h after drug treatments, and the data are presented as the mean ± SD of three independent experiments. *(p < 0.05) and **(p < 0.01), based on the differences between the indicated groups