Skip to main content
Fig. 2 | Parasites & Vectors

Fig. 2

From: ANISERP: a new serpin from the parasite Anisakis simplex

Fig. 2

Immunolocalization of ANISERP in sections of Anisakis simplex L3 with a rabbit hyperimmune serum. a Cross section at the level of the oesophagus showing strongly positive staining in SM and PF, moderate staining in SOM and slight staining of LC. No staining was observed in the EC. b The negative control incubated with the preimmune serum did not stain. c Cross section through the intestinal level showing in detail strongly positive immunostaining in the SM, which is restricted to the nSM; no staining was observed in the cSM, the C or EC, including the ca. Less intense staining was also observed in IC. d Section C was decolorized to remove the 4CN stain and subsequently stained with Wheatley’s trichrome. DOG, dorsal oesophageal gland; SOM, subventral muscular sectors of oesophagus; SM, somatic musculature; LC, lateral hypodermal cords; PF, pseudocoelomic fluid; EC, excretory cell; V, ventricle; IC, intestinal cells; nSM, non-contractile portion of somatic muscle cells (myocytons); cSM, contractile portion of somatic muscle cells; C, cuticle; ca, excretory canal; n, nucleus

Back to article page