From: ANISERP: a new serpin from the parasite Anisakis simplex
Enzyme | [Enzyme] | [Substrate] 250 μM | Activity buffer |
---|---|---|---|
Thrombin | 74 nM | Boc-Val-Arg -AMC | 50 mM Tris–HCl pH 8, 100 mM NaCl |
Trypsin | 54 nM | Boc-Gln-Ala-Arg-AMC | 50 mM Tris–HCl pH 8, 1 mM CaCl2, 0.15 M NaCl |
Cathepsin G | 0.33 nM | N-Succinyl-Ala-Ala-Phe-AMC. | 50 mM HEPES/NaOH pH 7.5 |
Cathepsin L | 0.83 nM | Z-Phe-Arg-AMC | Sodium acetate 100 mM pH 5.5, 1 mM EDTA, 4 mM DTT, 0.001% BSA |
Plasmine | 333 nM | Nt-Boc-Val-Leu-Lys-AMC | 50 mM Tris–HCl pH 8, 50 mM NaCl |
Elastase | 1.2 nM | N-Methoxysuccinyl-Ala-Ala-Pro-Val-7 amido AMC | 25 mM Tris–HCl pH 8, 100 mM NaCl, 1 mM CaCl2 |
Chymotrypsin | 0.012 nM | N-succinyl-Lelu-Leu-Val-Tyr-7 amido AMC | 100 mM HEPES/ NaOH pH 7.5 |
Factor Xa | 0.054 nM | Boc-Ile-Glu-Gly-Arg-7 amido AMC | 50 mM Tris–HCl pH 8.3, 5 mM CaCl2, 0.2 mM NaCl |
Factor XIa | 14,000 nM | Boc-Phe-Ser-Arg-7 amido AMC | 50 mM Tris–HCl pH 8, 100 mM NaCl, 1 mM CaCl2 |
Factor XIIa | 0.0017 nM | Boc-Val-Arg-AMC | 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 |