Fig. 5

Profiles of cercarial antigens recognized by domestic duck and mallard IgY and domestic duck IgM on Western blots. Only illustrative examples are shown. Lanes M - markers of MW. a - IgY response. Lane 1 - serum of a control domestic duck (group RD) 40 days after initiation of the experiment; lanes 2, 3, 4 - sera from a repeatedly infected domestic duck (group RD) 10, 20 and 30 days after first infection; lanes 5, 6 and 7 - serum of a domestic duck exposed to low dose of 100 cercariae (group LD) 20, 30 and 40 dpi; lane 8, 9 and 10 - sera of a “mature” mallard exposed once to 1500 cercariae (group SM) 20, 30 and 40 dpi. Arrowheads mark the proteins of 50, 47, 32 and 19 kDa that are specifically recognized by IgY. b - IgM response of domestic ducks exposed once to 1500 cercariae (group SD). Lanes 1, 2, 3, 4 and 5 - sera from a control duck 0, 10, 15, 20, 30 and 40 days after initiation of the experiment; lane 6 - serum of an experimental duck before infection; lanes 7, 8, 9 and 10 - sera of an infected duck 10, 15, 20, 30 and 40 dpi. Arrowheads point to the proteins of 75, 71, 50, 40, 37, 26, 20 and 17 kDa reacting with IgM from both control and infected ducks, and to the antigens of 64 and 62 kDa which were recognized specifically by IgM from the infected ducks only