Phytoecdysteroids as modulators of the Toxoplasma gondii growth rate in human and mouse cells

Parasites & Vectors

Table 2 Effect of phytoecdysteroids on the intensity of T. gondii proliferation in human PBMCs

Cmpd.	C [μg/mL]	Proliferation of parasites [%] in human PBMCs (N = 61)
		Toxo-seronegative (N = 40)		Toxo-seropositive (N = 21)
		[³H] uracil	qRT-PCR	[³H] uracil	qRT-PCR
α-ecdysone	2	90.59 ± 19.20	nt	86.04 ± 27.57	nt
	10	105.35 ± 25.33	nt	98.06 ± 21.55	nt
	20	^*115.09 ± 21.26	^*120.73 ± 27.06	^*140.74 ± 57.33	^*138.99 ± 51.02
20-hydroxy ecdysone	2	101.93 ± 23.22	nt	^*107.98 ± 22.54	nt
	20	^*117.23 ± 42.05	^*122.82 ± 30.58	^*111.68 ± 38.84	^*109.32 ± 26.28
	100	105.50 ± 32.48	nt	95.77 ± 21.85	nt
ConA	2.5	92.62 ± 29.98	nt	102.62 ± 25.35	nt

PBMCs isolated from individuals (N = 61) were tested in the presence of phytoecdysteroids and ConA in the concentration range between 2 and 100 μg/mL ± SD. Incubation period was 72 h. The statistically significant differences (p < 0.05) were labeled with an asterisk (*). To calculate the intensity of Toxoplasma proliferation compared to the untreated blank, the following equation was used: proliferation [%] = 100 × mean cpm incorporated into the treated cells in the [³H] uracil test or B1 gene in the qRT-PCR assay / mean cpm incorporated into the cultured cells or B1 gene without phytoecdysteroids

ISSN: 1756-3305