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Table 2 Effect of phytoecdysteroids on the intensity of T. gondii proliferation in human PBMCs

From: Phytoecdysteroids as modulators of the Toxoplasma gondii growth rate in human and mouse cells

Cmpd. C [μg/mL] Proliferation of parasites [%] in human PBMCs (N = 61)
Toxo-seronegative (N = 40) Toxo-seropositive (N = 21)
[3H] uracil qRT-PCR [3H] uracil qRT-PCR
α-ecdysone 2 90.59 ± 19.20 nt 86.04 ± 27.57 nt
10 105.35 ± 25.33 nt 98.06 ± 21.55 nt
20 *115.09 ± 21.26 *120.73 ± 27.06 *140.74 ± 57.33 *138.99 ± 51.02
20-hydroxy ecdysone 2 101.93 ± 23.22 nt *107.98 ± 22.54 nt
20 *117.23 ± 42.05 *122.82 ± 30.58 *111.68 ± 38.84 *109.32 ± 26.28
100 105.50 ± 32.48 nt 95.77 ± 21.85 nt
ConA 2.5 92.62 ± 29.98 nt 102.62 ± 25.35 nt
  1. PBMCs isolated from individuals (N = 61) were tested in the presence of phytoecdysteroids and ConA in the concentration range between 2 and 100 μg/mL ± SD. Incubation period was 72 h. The statistically significant differences (p < 0.05) were labeled with an asterisk (*). To calculate the intensity of Toxoplasma proliferation compared to the untreated blank, the following equation was used: proliferation [%] = 100 × mean cpm incorporated into the treated cells in the [3H] uracil test or B1 gene in the qRT-PCR assay / mean cpm incorporated into the cultured cells or B1 gene without phytoecdysteroids