Fig. 3

Toxic lectins bind to the gut of H. contortus. Indirect immunofluorescence microscopy using biotinylated lectins was performed on in situ labelled L1 stages and on fixed tissue sections of adults. All four toxic lectins (MOA, AAL, CCL2 and CGL2) bind to the digestive tract of larval stages distal from the oesophagus and to the brush border of adult gastrodermal cells. Additionally, CCL2 lectin also binds to the pharyngeal region of the L1 stage while MOA detects epitopes present in the inner cuticle layer of adults exposed by the sample preparation method. Tectonin does not show any fluorescent signal in the far-red channel (655λ) neither in larvae or adult stages, as well no detectable signal is present in the control larvae probed with Atto 655 Streptavidin alone. For an easier overview, the fluorescent signal from far-red channel is false-coloured differently for each lectin. Blue colour represents nuclear DNA stained with DAPI. DIC = Differential Interference Contrast