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Table 1 Universal and specific primers designed to detect microsporidian species

From: Invaders, natives and their enemies: distribution patterns of amphipods and their microsporidian parasites in the Ruhr Metropolis, Germany

Primer Sequence (5′–3′) Annealing Temperature Specificity
V1 CAC CAG GTT GAT TCT GCC TGA C 62 °C universal (Zhu et al. 1993, McClymont et al. 2005)
1342R ACG GGC GGT GTG TAC AAA GAA CAG
Dict11 F CGA CGT AAA CCT TTT GGT GCA R 60 °C Dictyocoela duebenum/ D. muelleri (Gr/GpC)
Dict11 R TYT CTT CCG CAA TAC TAA AAA ATT AAT AC
Dict14 F GGG CGA TTT ATT TGT TCT CCT GT 58 °C Dictyocoela duebenum/ D. muelleri (Gf/GpE)
Dict14 R GAT TTC TCT TCC GCA ATA CCA AAT YG
Dict16/17 F ATT GAT TAA RGA ACG AGC AGG GTT AG 62 °C Dictyocoela duebenum/ D. muelleri (GpC/Dh)
Dict16/17 R TCT TCC GCA AYA CMG CCA CA
Dict15 F TTT TAA TCG TGG CGT AAA CCA TK 62 °C D. berillonum
Dict15 R CTC TTC CGC AAT ACA GAA TAC CAT AC
Mic505 F CAT CAA CTA ACT TTG GGA AAC TAA G 62 °C Microsporidium sp. 505
Mic505 R TGG CCT CCC ACA CAT TCC GAG TG
Mic 515 F GGC GAT CTA ACC TCG GCA TCG GAT AAC C 62 °C Microsporidium sp. 515
Mic 515 R TGG CTT CCC ACC CAT TCC GAG C
Mic 3 F CAG TAA TGT TGC GAT GAT TTG GTC 58 °C Microsporidium sp. I
Mic 3 R CAG TAA ATA CTC CAC AGT ATC TTA C
Mic4 F TAC GGC TAA GAC GTG GAC 58 °C Microsporidium sp. nov. RR1
Mic4 R CAA TCC TAT TGC CAT CAT CTG
Mic6 F AGG ACC GAC GGC AAA GAA GTC 60 °C Microsporidium sp. nov. RR2
Mic6 R CAG GAG ATC TCA CCC ATT CAG
Mic7 F ACA GTT ATA ATT TAC TCG TAG ATC 58 °C Microsporidium sp. BPAR3
Mic7 R TAC TCG CAA GCA TGT GCT CA
Mic18/19 F ATA GAG GCG GTA GTA ATG AGA CGT A 58 °C C. dikerogammari/ Microsporidium sp. G
Mic18/19 R TTT AAC CAT AAA ATC ACT TCA CTC
  1. PCR program for all primers was: 98 °C for 3 min, 35 cycles of 98 °C-10 s, [annealing temp. see table]-10 s, 72 °C-15 s (10 s for Dict16/17, 20 s for V1/1342R) and final elongation at 72 °C for 3 min
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