Table 1 Universal and specific primers designed to detect microsporidian species

V1

CAC CAG GTT GAT TCT GCC TGA C

62 °C

universal (Zhu et al. 1993, McClymont et al. 2005)

1342R

ACG GGC GGT GTG TAC AAA GAA CAG

Dict11 F

CGA CGT AAA CCT TTT GGT GCA R

60 °C

Dictyocoela duebenum/ D. muelleri (Gr/GpC)

Dict11 R

TYT CTT CCG CAA TAC TAA AAA ATT AAT AC

Dict14 F

GGG CGA TTT ATT TGT TCT CCT GT

58 °C

Dictyocoela duebenum/ D. muelleri (Gf/GpE)

Dict14 R

GAT TTC TCT TCC GCA ATA CCA AAT YG

Dict16/17 F

ATT GAT TAA RGA ACG AGC AGG GTT AG

62 °C

Dictyocoela duebenum/ D. muelleri (GpC/Dh)

Dict16/17 R

TCT TCC GCA AYA CMG CCA CA

Dict15 F

TTT TAA TCG TGG CGT AAA CCA TK

62 °C

D. berillonum

Dict15 R

CTC TTC CGC AAT ACA GAA TAC CAT AC

Mic505 F

CAT CAA CTA ACT TTG GGA AAC TAA G

62 °C

Microsporidium sp. 505

Mic505 R

TGG CCT CCC ACA CAT TCC GAG TG

Mic 515 F

GGC GAT CTA ACC TCG GCA TCG GAT AAC C

62 °C

Microsporidium sp. 515

Mic 515 R

TGG CTT CCC ACC CAT TCC GAG C

Mic 3 F

CAG TAA TGT TGC GAT GAT TTG GTC

58 °C

Microsporidium sp. I

Mic 3 R

CAG TAA ATA CTC CAC AGT ATC TTA C

Mic4 F

TAC GGC TAA GAC GTG GAC

58 °C

Microsporidium sp. nov. RR1

Mic4 R

CAA TCC TAT TGC CAT CAT CTG

Mic6 F

AGG ACC GAC GGC AAA GAA GTC

60 °C

Microsporidium sp. nov. RR2

Mic6 R

CAG GAG ATC TCA CCC ATT CAG

Mic7 F

ACA GTT ATA ATT TAC TCG TAG ATC

58 °C

Microsporidium sp. BPAR3

Mic7 R

TAC TCG CAA GCA TGT GCT CA

Mic18/19 F

ATA GAG GCG GTA GTA ATG AGA CGT A

58 °C

C. dikerogammari/ Microsporidium sp. G

Mic18/19 R

TTT AAC CAT AAA ATC ACT TCA CTC