Fig. 3

Impairment of parasitic motility impeded NO-induced egress of T. gondii from HFFs. NO-induced egress after pre-treatment with cytochalasin D (a) or DMEM (b) was observed by microscopy. Intact parasitophorous vacuole (black arrow), lytic parasitophorous vacuole (white arrow). Bar: 20 μm. c Tachyzoite- infected HFFs were pre-treated with cytochalasin D before NO-induced egress assay. Egressed parasites were suspended with 500 μL of DMEM, and 20 μL suspensions were analysed by flow cytometry. The data in the figure show the means ± SEMs of five replicates and represent three independent experiments, **P < 0.01