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Fig. 2 | Parasites & Vectors

Fig. 2

From: Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcIDOM)

Fig. 2

Molecular typing for cultured parasites and parasite clones. DNA was extracted from epimastigote hemocultures (HC) and five derived clones (CL1-CL5). TcI-TcVI correspond to DTU controls, NC corresponds PCR negative control. Lanes containing restriction products are labeled with an asterisk (*). Only restriction products are shown for controls. DNA was analyzed by the PCR-RFLP scheme proposed by Lewis, et al., 2009 [12]: as indicated by the brackets on the right side, fragments from the LSUrDNA, HSP60 and GPI genes were amplified by PCR. GPI and HSP60 products were digested with HhaI and EcoRV restriction enzimes, respectively

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