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Fig. 1 | Parasites & Vectors

Fig. 1

From: Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection

Fig. 1

a Agarose gel image of the Dra1 RPA amplicons. Low limit of detection can be seen at 100 fg of S. haematobium gDNA. b LF strips showing the detection of the Dra LF-RPA amplicons. Lower limit of detection can be seen at 100 fg of S. haematobium gDNA. NC = Negative control. The LF-RPA oligonucleotides consisted of a forward primer, a specialised 6-FAM labelled oligonucleotide probe and a reverse biotin labelled primer. Upon successful binding to the complementary gDNA target, amplification ensues resulting in the formation of a double-labelled amplicon. When run on an oligochromatographic LF strip, the amplicon binds to anti-FAM antibodies and antibody labelled gold colloid nanoparticles in the running buffer bind to the biotin antigen resulting in a semi quantitative colour change. The LF strip also has a control line to test for reaction failure

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