Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection

Table 1 Primers / probe sequence design for the Dra1 RPA and LF assays

Assay	Name	Sequence (5′-3′)
RPA	Dra1-F1	ATCTCACCTATCAGACGAAACAAAGAAAAT
RPA	Dra1-R1	AATATGAAACAATTTTCACAACGATACGAC
LF-RPA	Dra1-F1-LF	ATCTCACCTATCAGACGAAACAAAGAAAAT
	Dra1-R1-LF	(Z)AATATGAAACAATTTTCACAACGATACGAC
	Dra1 LF probe	(Y)AATTGTTGGTGGAAGTGCCTGTTTCGCAAT(H)TCTCCGGAATGGTTG(3)

Features: Z = Biotin label; Y = 6-carboxyfluorescein (FAM) label; H = abasic tetrahydrofuran (THF) residue; 3 = C3 spacer. All primers were designed according to the instructions from TwistDx (http://www.twistdx.co.uk). The Dra1 LF probe was designed with a recommended length of 46–52 bp with at least 30 bp placed 5′ to the abasic tetrahydrofuran (THF) residue (H). The RPA and LF RPA primers are the same but with a 5′ modification on the reverse primer (Dra1-R1-LF) for the LF RPA assay

ISSN: 1756-3305