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Table 2 Results showing the impact of crude urine on the efficacy of the Dra1 RPA-LF assay and subsequent LF detection. All reactions were performed at 37 °C for 20 mins

From: Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection

Measure of crude urine in each Dra1 RPA-LF reaction Dra1 RPA-LF efficacy
Volume of crude urine (μl) % of crude urine Amount of S. haematobium gDNA
10 pg 100 fg
0.31 1.25 % +++ +++
0.63 2.5 % +++ ++
1.25 5 % +++ +
2.50 10 % +++ -
  1. The number of + refers to the qualitative signal on the LF test line. +++ = very strong and - = no trace