Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection

Table 2 Results showing the impact of crude urine on the efficacy of the Dra1 RPA-LF assay and subsequent LF detection. All reactions were performed at 37 °C for 20 mins

Measure of crude urine in each Dra1 RPA-LF reaction		Dra1 RPA-LF efficacy
Volume of crude urine (μl)	% of crude urine	Amount of S. haematobium gDNA
Volume of crude urine (μl)	% of crude urine	10 pg	100 fg
0.31	1.25 %	+++	+++
0.63	2.5 %	+++	++
1.25	5 %	+++	+
2.50	10 %	+++	-

The number of + refers to the qualitative signal on the LF test line. +++ = very strong and - = no trace

ISSN: 1756-3305