Fig. 2

BSR monolayers were infected with BTV1[VP2]2 or DISA 2 and immunostained with α NS3 or VP7 MAbs. Clearly, VP7 is expressed in both infections (red colour), whereas NS3/NS3a is only detectable in the BTV1[VP2]2 infected cells (a). Release of BTV1[VP2]2 and DISA 2 virus on BSR cells (b) and KC cells (c) was also examined. Both virus in the cell fraction (black) and released virus (grey) was measured. Experiments were performed in duplicate and error bars represent SEM