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Table 4 Comparison of presence of Blastocystis and/or gastrointestinal symptoms to specific sized antibody bands directed against Blastocystis proteins using Western immunoblotting

From: Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls

  

Presence of antibody to Blastocystis protein at band MW (n, %)

Gp1

Gp2

17 kDa

27 kDa

37 kDa

50 kDa

60-65 kDa

75-90 kDa

95-105 kDa

150 kDa

  

Gp1

Gp2

Gp1

Gp2

Gp1

Gp2

Gp1

Gp2

Gp1

Gp2

Gp1

Gp2

Gp1

Gp2

Gp1

Gp2

Blastocystis presence

                

IBS-P & HC-P (n = 50)

IBS-N & HC-N (n = 30)

6 (12)

0 (0)*

42 (84)

19 (63)*

6 (12)

0 (0)*

42 (84)

21 (70)

45 (90)

25 (83)

39 (78)

22 (73)

28 (56)

5 (17)***

21 (42)

2 (7)***

IBS-P & HC-P (n = 50)

HC-N (n = 16)

6 (12)

0 (0)

42 (84)

7 (44)***

6 (12)

0 (0)

42 (84)

8 (50)**

45 (90)

13 (81)

39 (78)

9 (56)

28 (56)

2 (13)**

21 (42)

1 (6)**

IBS-P (n = 26)

IBS-N (n = 14)

1 (4)

0 (0)

25 (96)

12 (86)

3 (12)

0 (0)

26 (100)

13 (93)

26 (100)

12 (86)*

25 (96)

13 (93)

14 (54)

3 (21)*

5 (19)

1 (7)

HC-P (n = 24)

HC-N (n = 16)

5 (21)

0 (0)^

17 (71)

7 (44)

3 (13)

0 (0)

16 (67)

8 (50)

19 (79)

13 (81)

14 (58)

9 (56)

14 (58)

2 (13)**

16 (67)

1 (6)***

GIT symptoms

                

IBS-P & IBS-N (n = 40)

HC-P & HC-N (n = 40)

1 [56]

5 (13)

37 (93)

24 (60)***

3 (8)

3 (8)

39 (98)

24 (60)***

38 (95)

32 (80)*

38 (95)

23 (58)***

17 (43)

16 (40)

6 (15)

17 (43)**

IBS-P (n = 26)

HC-P & HC-N (n = 40)

1 (4)

5 (13)

25 (96)

24 (60)***

3 (12)

3 (8)

26 (100)

24 (60)***

26 (100)

32 (80)*

25 (96)

23 (58)***

14 (54)

16 (40)

5 (19)

17 (43)*

IBS-P (n = 26)

HC-P (n = 24)

1 (4)

5 (21)

25 (96)

17 (71)*

3 (12)

3 (13)

26 (100)

16 (67)***

26 (100)

19 (80)*

25 (96)

14 (58)***

14 (54)

14 (58)

5 (19)

16 (67)***

IBS-P (n = 26)

HC-N (n = 16)

1 (4)

0 (0)

25 (96)

7 (44)***

3 (12)

0 (0)

26 (100)

8 (50)***

26 (100)

13 (81)*

25 (96)

9 (56)***

14 (54)

2 (13)**

5 (19)

1 (6)

IBS-N (n = 14)

HC-P & HC-N (n = 40)

0 (0)

5 (13)

12 (86)

24 (60)

0 (0)

3 (8)

13 (93)

24 (60)*

12 (86)

32 (80)

13 (93)

23 (58)*

3 (21)

16 (40)

1 (7)

17 (43)*

IBS-N (n = 14)

HC-N (n = 16)

0 (0)

0 (0)

12 (86)

7 (44)*

0 (0)

0 (0)

13 (93)

8 (50)*

12 (86)

13 (81)

13 (93)

9 (56)*

3 (21)

2 (13)

1 (7)

1 (6)

  1. Gp: group of study subjects; n: number of subjects; kDa: kiloDalton; MW: molecular weight
  2. IBS-P/N: Irritable bowel syndrome subjects positive/negative for Blastocystis
  3. HC-P/N: healthy control subjects positive/negative for Blastocystis
  4. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001
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