Fig. 4

nsP3 interacts with the NTF2-like domain of mosquito Rasputin. a Protein alignment of the Rin/G3BP NTF2-like domains from Ae. albopictus (KP641128), Ae. aegypti, (XP_001651045), Culex quincefasciatus (XP_001861860), Anopheles gambiae (XP_001688309), Anopheles sinensis (KFB40464), Drosophila melanogaster (AF231031), Ixodes ricinus (GANP01009274), and Homo sapiens (CAG38772). Genbank accession numbers in brackets. Alignment made with CLUSTALX and modified using Genedoc. b Structural modeling of Ae. albopictus-Rin modeled onto D. melanogaster Rin. Modeling was performed using the Phyre2 server (www.sbg.bio.ic.ac.uk/phyre2), results were visualized with PyMOL (www.pymol.org). Left, Ae. albopictus Rin (deep blue) is depicted together with D. melanogaster Rin (cyan). Right, the FxFG binding pocket is shown and phenylalanine 34 is highlighted in red. c Sf21 insect cells were co-transfected with EGFP-nsP3 and either Rin-mCherry (wild type), Rin-mCherry F34A or Rin-mCherry F34W