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Fig. 5 | Parasites & Vectors

Fig. 5

From: Mosquito Rasputin interacts with chikungunya virus nsP3 and determines the infection rate in Aedes albopictus

Fig. 5

Formation of nsP3-granules is independent of mosquito Rasputin. a. Rin silencing was determined by semi-quantitative RT-PCR on Ae. albopictus Rin mRNA, relative for the internal control S7. Bars represent relative Rin mRNA expression normalized to dsLuc transfected samples. Error bars indicate standard deviation of duplicate samples from a single representative experiment, which is presented in B. b. U4.4 mosquito cells were transfected with dsRNA against Ae. albopictus Rin or luciferase. Twenty-four hours later these cells were transfected with wild type EGFP-nsP3, displaying a granular localization

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