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Fig. 7 | Parasites & Vectors

Fig. 7

From: Mosquito Rasputin interacts with chikungunya virus nsP3 and determines the infection rate in Aedes albopictus

Fig. 7

CHIKV infection in vitro is not affected by Rin depletion. In four independent experiments, U4.4 mosquito cells were transfected with dsRNA against Ae. albopictus Rin or luciferase. Twenty-four hours later these cells were infected with CHIKV (MOI 5) and total RNA was isolated at 16 or 24 hpi. a Rin silencing was determined by semi-quantitative RT-PCR on Ae. albopictus Rin mRNA, normalized for the internal control S7. b CHIKV genomic RNA was quantified with primers that anneal to the nsP1 gene. c CHIKV structural protein expression was determined by immunoblot staining against CHIKV E2 and host cell β-tubulin. Protein sizes indicated in kDa. Results of duplicate experiments are shown. d At the indicated hpi medium was harvested and the CHIKV titer (TCID50/ml) was determined in end point dilution essays. A,B,D. Bars represent the mean of four independent experiments, which have been normalized to the respective value of dsLuc transfected samples in each individual experiment. Error bars represent one standard error of the mean

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