Table 1 Oligonucleotides used in this study
Name | Sequence |
|---|---|
attB2-R-phos | TCATACCCAGCTTTCTTGTAC |
CHIKVnsP3-2R | TCAGCGTGATGGCACGTTATGG |
CHIKV nsP3-DDEL-R | TCATAATTCGTCGTCCGTGTC |
CHIKV nsP3-dUGA-R | TCACCCACCTGCCCTATCTAGTAATTCGTCGTCCGTGTCTG |
EGFP-R-phos | CTTGTACAGCTCGTCCATG |
EcoRI-nsP3-161-F | CAAGTGGAATTACTAGACGAAC |
nsP3-319-F | GTTAGTCCAAGGGAATATAAATC |
Aalb-Rin-F | ATGGTAATGGAAGCACAACC |
Aalb-Rin-R | CTAACGTCGTCCTCCGTAG |
Aalb-Rin-intF | TCCAAGTGTCGCTACC |
HindIII-EcoRI-Aalb-Rin-F | GATAAGCTTGAATTCACCATGGTAATGGAAGCACAACC |
HindIII-Aalb-Rin-R | GATAAGCTTACGTCGTCCTCCGTAGG |
nsP3-FG479AA F | TAACGGCCGCGGATTTTGATGAAGGGGAGA |
nsP3-FG479AA R | AATCCGCGGCCGTTATGGGGAAAGTCTCGT |
nsP3-FG497AA F | TGACCGCTGCGGACTTCTCGCCGGGCGAAG |
nsP3-FG497AA R | GTCCGCAGCGGTCAGTAACTCAGAGGACAA |
Rin-F34A F | GCACCGTGCCTACAACAACTCGTCGAGCTTC |
Rin-F34A R | TGTTGTAGGCACGGTGCAGGTGATCCGGCG |
Rin-F34A F | GCACCGTTGGTACAACAACTCGTCGAGCTTC |
Rin-F34A R | TGTTGTACCAACGGTGCAGGTGATCCGGCG |
T7-pGEM-Teasy-R | TAATACGACTCACTATAGGGGCCGCGAATTCACTAGTG |
Aalb-Rin F2 | GTATGCCAACCATTGATCCG |
Aalb-Rin R2 | GTCCAGTTCGTTCATTGACAG |
nsP1-int F | CTGACGGAAGGTAGACGAG |
nsP1-int R2 | GCACGTGAAGCTGAGCTTCCC |
S7 F | CCAGGCTATCCTGGAGTTG |
S7 R | GACGTGCTTGCCGGAGAAC |
nsP3-P398A F | GAATACCGCGGCAGTCGCA |
nsP3-P398A R | TGCGACTGCCGCGGTATTC |
nsP3-PPR401AAA F | CGCCAGTCGCAGCGGCCGCAAGAAGACGTGGGAA |
nsP3-PPR401AAA R | CCCACGTCTTCTTGCGGCCGCTGCGACTGGCGCGGTAT |