Fig. 1

Detection of Plasmodium ookinetes in the mosquito midgut. a and b Representatitve images of smears of midgut contents stained with Giemsa from An. coluzzii mosquitoes infected with P. berghei (a) and P. yoelii (b). Midguts were dissected at 18, 24 and 48 h post infection (hpi). Arrowheads show zygotes/ookinetes (black), bacteria (red), remains of dead parasites (white) and blood meal contents (yellow). c and d Agarose gel images showing SOAP gene transcripts amplified from gut contents of mosquitoes infected with P. berghie (c) or P. yoelii (d). Midguts were dissected at 0, 6, 18, 24 and 48 hpi. The An. gambiae S7 gene served as a heterologous internal standard. M corresponds to molecular marker