Fig. 3From: Detection of viable Plasmodium ookinetes in the midguts of Anopheles coluzzi using PMA-qrtPCRDifferential staining of live and dead ookinetes and bacteria in the mosquito midgut. a Heat killed ookinetes an overnight ookinete culture stained with a mixture of PMA and SYBR solution. b Live and dead parasites from an overnight ookinete culture stained with a mixture of PMA and SYBR solution. c Live and dead P. berghei ookinetes in the mosquito midgut epithelium at 48 hpi. d Live and dead bacteria present in midgut contents. Live cells are stained with SYBR green (white arrowheads), while dead cells are stained with PMA (red arrowheads)Back to article page