Fig. 1

Annexin A2 expression is required during host cell invasion by T. cruzi. a, b: 5x10⁴ wild-type and Annexin A2 knockout murine embryonic fibroblasts (MEF) (a gift from Prof. Dr. Stephen E. Moss, Institute of Ophthalmology, University College London)/ well were seeded into 24 well plates overnight. Cells were infected with T. cruzi G-strain EAs for one hour at a multiplicity of infection (MOI) of five parasites/cell. Cells were then washed with PBS, fixed with Bouin and stained with Giemsa. The results of three independent experiments performed in triplicate are shown. The number of internalized parasites was determined by counting 200 cells/coverslip. 200 infected cells/coverslip were counted in multiplication assays. Significant differences were determined by GraphPad Prism software, version 5.01 and Student’s t-test was used. Differences were considered significant when p < 0.05