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Table 2 Kinetic parameters for the CO2 hydration reaction catalysed by the human isozymes hCA I, II, III, IV, VA, VB, VI, VII, IX, XII, XIII, and XIV (α-class CAs) and the β-CAs from Leishmania donovani chagasi (LdcCA), Drosophila melanogaster (DmBCA), Anopheles gambiae (AgaCA) and Ascaris lumbricoides (AlBCA)

From: Ascaris lumbricoides β carbonic anhydrase: a potential target enzyme for treatment of ascariasis

Enzyme

Class

kcat (s−1)

kcat/Km (M−1x s−1)

Ki (acetazolamide) (nM)a

hCA I

α

2.0×105

5.0×107

250 ± 12

hCA II

α

1.4×106

1.5×108

12 ± 0.8

hCA III

α

1.3×104

2.5×105

240000 ± 25000

hCA IV

α

1.1×106

5.1×107

74 ± 5.5

hCA VA

α

2.9×105

2.9×107

63 ± 2.1

hCA VB

α

9.5×105

9.8×107

54 ± 3.0

hCA VI

α

3.4×105

4.9×107

11 ± 0.7

hCA VII

α

9.5×105

8.3×107

2.5 ± 0.11

hCA IX

α

1.1×106

1.5×108

16 ± 0.8

hCA XII

α

4.2×105

3.5×107

5.7 ± 0.04

hCA XIII

α

1.5×105

1.1×107

16 ± 0.3

hCA XIV

α

3.1×105

3.9×107

41 ± 2.2

LdcCA

β

9.35×105

5.9 ×107

91.7 ± 5.7

DmBCA

β

9.5×105

1.1×108

516 ± 24

AgaCA

β

7.2×105

5.6×107

27.3 ± 2.0

AlBCAa

β

(6.0 ± 0.1) ×105

(4.3 ± 0.2) ×107

84.1 ± 2.9

  1. aMean ± standard error from three different assays