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Table 2 Kinetic parameters for the CO2 hydration reaction catalysed by the human isozymes hCA I, II, III, IV, VA, VB, VI, VII, IX, XII, XIII, and XIV (α-class CAs) and the β-CAs from Leishmania donovani chagasi (LdcCA), Drosophila melanogaster (DmBCA), Anopheles gambiae (AgaCA) and Ascaris lumbricoides (AlBCA)

From: Ascaris lumbricoides β carbonic anhydrase: a potential target enzyme for treatment of ascariasis

Enzyme Class kcat (s−1) kcat/Km (M−1x s−1) Ki (acetazolamide) (nM)a
hCA I α 2.0×105 5.0×107 250 ± 12
hCA II α 1.4×106 1.5×108 12 ± 0.8
hCA III α 1.3×104 2.5×105 240000 ± 25000
hCA IV α 1.1×106 5.1×107 74 ± 5.5
hCA VA α 2.9×105 2.9×107 63 ± 2.1
hCA VB α 9.5×105 9.8×107 54 ± 3.0
hCA VI α 3.4×105 4.9×107 11 ± 0.7
hCA VII α 9.5×105 8.3×107 2.5 ± 0.11
hCA IX α 1.1×106 1.5×108 16 ± 0.8
hCA XII α 4.2×105 3.5×107 5.7 ± 0.04
hCA XIII α 1.5×105 1.1×107 16 ± 0.3
hCA XIV α 3.1×105 3.9×107 41 ± 2.2
LdcCA β 9.35×105 5.9 ×107 91.7 ± 5.7
DmBCA β 9.5×105 1.1×108 516 ± 24
AgaCA β 7.2×105 5.6×107 27.3 ± 2.0
AlBCAa β (6.0 ± 0.1) ×105 (4.3 ± 0.2) ×107 84.1 ± 2.9
  1. aMean ± standard error from three different assays