Fig. 2

ROP18 induced the apoptosis of N2a cells. aThe N2a cells were transfected with ROP18-GFP, 14-3-3-GFP or control GFP vector for 24 h. Then the apoptosis of the cells were detected by flow cytometry after Annexin V-PE/7-AAD staining. b The N2a cells were infected with ROP18-RH, RH tachyzoites at an m.o.i of ~3 or treated with Apopida (apoptosis inducer A) for 24 h. Then the apoptosis of the cells were detected by flow cytometry after Annexin V-PE/7-AAD staining. The plots are from a representative measurement and the quantitative data were expressed as mean ± SD on three different assays (n = 3). *P < 0.05; **P < 0.01; * **P < 0.001 vs. Negative controls