Fig. 1

Proliferation Curve of Vero Cells. The cells that were seeded in E-Plate 96 and were constantly observed by measuring CI values to obtain the ideal cell number and to distinguish the suitable time point for virus infection prior to the antiviral assay are indicated. Cell features such as adhesion, spreading and proliferation were observed in intervals of 2 minutes. The dash line marks the initial phase of cell adhesion and spreading determined by the CI curve of 1.8 × 10⁴ cells at 18 h. Coloured curves represent the various numbers of cells seeded per well in E-Plate 96: Blue line: 2.0 × 10⁴ cells/well; Pink line: 1.8 × 10⁴ cells/well; Turquoise line: 1.5 × 10⁴ cells/well. Each data point signifies the average ± standard deviation and was analysed in triplicate