Pathogen
|
Assays
|
Oligonucleotide sequences (5' > 3')
|
Product size (bp)
|
Reference
|
---|
Target gene
|
---|
B. bovis SBP-4
|
PCR
|
AGTTGTTGGAGGAGGCTAAT
|
907
|
[21]
|
TCCTTCTCGGCGTCCTTTTC
|
nPCR
|
GAAATCCCTGTTCCAGAG
|
503
|
TCGTTGATAACACTGCAA
|
B. bigemina RAP-1a
|
PCR
|
GAGTCTGCCAAATCCTTAC
|
879
|
TCCTCTACAGCTGCTTCG
|
nPCR
|
AGCTTGCTTTCACAACTCGCC
|
412
|
TTGGTGCTTTGACCGACGACAT
|
semi nPCR
|
GAGTCTGCCAAATCCTTAC
|
690
|
TTGGTGCTTTGACCGACGACAT
|
Theileria spp. 18S rRNA
|
PCR
|
GAAACGGCTACCACATCT
|
778
|
[20]
|
AGTTTCCCCGTGTTGAGT
|
nPCR
|
TTAAACCTCTTCCAGAGT
|
581
|
TCAGCCTTGCGACCATAC
|
T. parva p 104
|
PCR
|
ATTTAAGGAACCTGACGTGACTGC
|
496
|
[23]
|
TAAGATGCCGACTATTAATGACACC
|
nPCR
|
GGCCAAGGTCTCCTTCAGATTACG
|
277
|
TGGGTGTGTTTCCTCGTCATCTGC
|
T. orientalis MPSP
|
PCR
|
CTTTGCCTAGGATACTTCCT
|
776
|
[24]
|
ACGGCAAGTGGTGAGAACT
|
A. marginale Msp5
|
PCR
|
GTGTTCCTGGGGTACTCCTATGTGAACAAG
|
547
|
[22]
|
AAGCATGTGACCGCTGACAAACTTAAACAG
|
nPCR
|
AAGCACATGTTGGTAATATTCGGCTTCTCA
|
195
|
AATTCTCGCATCAAAAGACTTGTGGTACTC
|
-
Note: The primers sets forSBP-4, 18S rRNA, p104 and MPSP genes were used for detection of corresponding pathogens and the products of the last amplification served as template for genetic characterisation. With regard to BbigRAP-1a and Msp5, PCR and nPCR primers were used in pathogen detection, however for genetic characterization, amplicons from a semi-nPCR (BbigRAP-1) and from the first PCR (Msp5) were used