Molecular detection and characterization of Babesia bovis , Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya

Table 1 Sequences of primers set used for detection of hemoparasites DNAs

Pathogen	Assays	Oligonucleotide sequences (5' > 3')	Product size (bp)	Reference
Target gene	Assays	Oligonucleotide sequences (5' > 3')	Product size (bp)	Reference
B. bovis SBP-4	PCR	AGTTGTTGGAGGAGGCTAAT	907	[21]
	PCR	TCCTTCTCGGCGTCCTTTTC	907
	nPCR	GAAATCCCTGTTCCAGAG	503
	nPCR	TCGTTGATAACACTGCAA	503
B. bigemina RAP-1a	PCR	GAGTCTGCCAAATCCTTAC	879
	PCR	TCCTCTACAGCTGCTTCG	879
	nPCR	AGCTTGCTTTCACAACTCGCC	412
	nPCR	TTGGTGCTTTGACCGACGACAT	412
	semi nPCR	GAGTCTGCCAAATCCTTAC	690
	semi nPCR	TTGGTGCTTTGACCGACGACAT	690
Theileria spp. 18S rRNA	PCR	GAAACGGCTACCACATCT	778	[20]
	PCR	AGTTTCCCCGTGTTGAGT	778
	nPCR	TTAAACCTCTTCCAGAGT	581
	nPCR	TCAGCCTTGCGACCATAC	581
T. parva p 104	PCR	ATTTAAGGAACCTGACGTGACTGC	496	[23]
	PCR	TAAGATGCCGACTATTAATGACACC	496
	nPCR	GGCCAAGGTCTCCTTCAGATTACG	277
	nPCR	TGGGTGTGTTTCCTCGTCATCTGC	277
T. orientalis MPSP	PCR	CTTTGCCTAGGATACTTCCT	776	[24]
T. orientalis MPSP	PCR	ACGGCAAGTGGTGAGAACT	776	[24]
A. marginale Msp5	PCR	GTGTTCCTGGGGTACTCCTATGTGAACAAG	547	[22]
	PCR	AAGCATGTGACCGCTGACAAACTTAAACAG	547
	nPCR	AAGCACATGTTGGTAATATTCGGCTTCTCA	195
	nPCR	AATTCTCGCATCAAAAGACTTGTGGTACTC	195

Note: The primers sets forSBP-4, 18S rRNA, p104 and MPSP genes were used for detection of corresponding pathogens and the products of the last amplification served as template for genetic characterisation. With regard to BbigRAP-1a and Msp5, PCR and nPCR primers were used in pathogen detection, however for genetic characterization, amplicons from a semi-nPCR (BbigRAP-1) and from the first PCR (Msp5) were used