Table 2 Primers used in nested PCR reactions in multilocus fragment typing of cattle-derived Cryptosporidium parvum

MM5

1°

TCACAAGTTACCCCTTCTGATGCTG

TCCACCTCCGGATTGGTTGTG

[18]

2°

CCTGGACTTGGATTTGGACTTACACC

GGAGAAGATAAGCTAGCCGAATCT

[12]

MM18

1°

GTTCAGCTGATACGGGTTTGCAACA

CATCACCATCTCCTCCGCCAGA

[18]

2°

CTTTCTGGAGGGTTTGTTCCTCC

CTTCCTGATGATCCAGGCCAAGC

[12]

MM19

1°

TGGTTTTAGCTAAGGAAGCGATAG

CTGCTGCTGCTGTTGCTTTA

[18]

2°

GATTCTGTCAACTTTGAATTCAG

CCAACCCCGAATTCATTTCCAAC

[12]

TP14

1°

GAGAAGGAGCAATGGGAGCA

TCCTCCTTTTTGCCCTTGAA

[18]

2°

CTAACGTTCACAGCCAACAGTACC

CAATAAAGACCATTATTACCACC

[20]

MS1

1°

AAGGGTGAGGATGAGCAGAA

TTCTTAACTTTCCATTTTGAGTGA

Current study

2°

TTAGTCGACCTCTTCAACAGTTGG

GGAACACCATCCAAGAACCAAAGGT

[26]

MS9

1°

TTAGTCGACCTCTTCAACAGTTGG

CAGAAT TGGAATCATTTTCTGAAT

Current study

2°

GGACTAGAAATAGAGCTTTGGCTGG

GTCTGAGACAGAATCTAGGATCTAC

[19]

gp60

1°

ATAGTCTCCGCTGTATTC

GAGATATATCTTGGTGCG

[7]

2°

TCCGCTGTATTCTCAGCC

CGAACCACATTACAAATGAAG

[7]