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Fig. 1 | Parasites & Vectors

Fig. 1

From: A Toxoplasma gondii vaccine encoding multistage antigens in conjunction with ubiquitin confers protective immunity to BALB/c mice against parasite infection

Fig. 1

Construction of DNA and adenovirus vaccines encoding ubiquitin-conjugated multistage antigen segments derived from T. gondii. a Schematic representation of the compound proteins, which encode amino acid sequences of eight protein segments derived from tachyzoite, bradyzoite and sporozoite antigens of T. gondii. b Construction of recombinant DNA and adenovirus vaccines expressing ubiquitin-conjugated multistage antigen segments. The recombinant eukaryotic plasmids pVAX1-MAS (c), pVAX1-UMAS (d), and pHBAd-MCMV-GFP-UMSA (e) were identified by restriction enzyme digestion analysis (Lane 1). Lane M: DNA marker. f Recombinant adenoviral particles were obtained from 293 cells

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