Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia

Parasites & Vectors

Table 2 Detections of trypanosome species in tsetse flies from Chama district by PCR and LAMP

Tsetse fly species			SRA-PCR		KIN-PCR				TviCatL-PCR
			SRA-PCR		Trypanozoon		T. congolense		TviCatL-PCR
			+	_-	+	_-	+	_-	+	_-
G. m. morsitans	PFR-LAMP	+	16	36	8^a	44	10^b	42	11^c	41
(females)		_-	0	413	0	413	45	368	94	319
G. m. morsitans		+	3^d	0	1^e	2	1^e	2	3^d	0
(males)		-	0	24	0	24	1	23	10	14
G. pallidipes		+	0	0	0	0	0	0	0	0
(males)		-	0	54	0	54	4^f	50	19^g	35

^aTwo samples were SRA-PCR positive. ^bSix samples were positive for Trypanozoon by KIN-PCR and PFR-LAMP; 3 SRA- PCR positive; 1 TviCatL-PCR positive. ^cTwo samples were SRA-PCR positive. ^dThese were same samples. ^eThis was the same SRA-PCR and TviCatL-PCR positive sample. ^fOne sample tested positive for both T. congolense Savannah and Kilifi type. ^gOne sample also tested positive for T. congolenseSavannah type

ISSN: 1756-3305