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Fig. 1 | Parasites & Vectors

Fig. 1

From: Development of a generic Ehrlichia FRET-qPCR and investigation of ehrlichioses in domestic ruminants on five Caribbean islands

Fig. 1

Alignment of the primers and probes of the generic Ehrlichia FRET-qPCR with the 16S rRNA gene sequences of Ehrlichia spp. and related genera/species. The sequences of the upstream/downstream primers and the fluorescein/LCRed 640 probes are shown at the top of the boxes. The upstream primer and two probes were used as the indicated sequences while the downstream primer was used as an antisense oligonucleotide. The 6-FAM label was attached directly to the 3-terminal nucleotide of the fluorescein probe and the LCRed 640 fluorescein label was added via a linker to the 5′-end of the LCRed 640 probe. Dots indicate nucleotides identical to the primers and probes, and dashes denote the deletion of a nucleotide. Both of the primers and the fluorescein probe had 100 % identity with all Ehrlichia spp. while the LCRed probe had 0, 1, 2 or 3 nucleotide mismatches. The primers and probes had multiple mismatches with other related organisms

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