Fig. 10

Effects of vaccination of rSjCa8 on NO production in the skin and on the number of invaded larvae. Photomicrographs (×40) of recovered schistosomula from different experimental groups as observed using a microscope (a). Harvested larvae of S. japonicum were counted (b), and NO concentrations in the skin homogenates were determined by absorbance at a 540-nm wavelength using a total nitric oxide assay kit; **P < 0.01, ***P < 0.001 compared with uninfected mice; ## P < 0.01, ###P < 0.001 compared with the adjuvant/infected group; ▲▲ P < 0.01 compared with the adjuvant + rSjCa8/infected group. Experiments were replicated three times