Fig. 5

Inhibition of NO release by LPS-stimulated RAW264.7 cells treated with rSjCa8. RAW264.7 cells were cultured in medium containing 1 μg/ml LPS for 24 h, and were subsequently exposed for another 30 min to PBS, rSjCa8 (1 or 20 μg/ml), or rSj13 (20 μg/ml). NO generation by the cells that were loaded with the NO-specific fluorescent probe DAF-AM was detected as intracellular green fluorescence signal by laser-scanning confocal microscopy